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empty vector control hek293 cells  (Addgene inc)


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    Addgene inc empty vector control hek293 cells
    Empty Vector Control Hek293 Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+empty+vector+control/pLJM1-EGFP+(Plasmid+%2319319)/pm37816711-338-9-18
    Average 96 stars, based on 405 article reviews
    empty vector control hek293 cells - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. Vector construction and cell transfection The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Article Title: Immune-evasion of KRAS -mutant lung adenocarcinoma mediated by cAMP response element-binding protein
    Article Snippet: B16F10 p Kras G12C , PANO2 p Kras G12C and HEK293T p Kras G12C cells were generated after stable transfection of B16F10, PANO2 and HEK293T cells with plasmids eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372), eGFP.KRASG12C-2A.retro.puro (Addgene ID64373, RRID:Addgene_64373) and eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372) respectively as described elsewhere ( ). .. B16F10 p C and PANO2 p C were stably transfected with control (empty) overexpression vector eGFP.retro.puro (Addgene ID 64336, RRID:Addgene_64336) ( ). .. HEK293T p Kras G12C were stably transfected with p LUC (Cag.Luc.puro, Addgene ID 74409, RRID:Addgene_74409) or with plasmid enconding Creb1 (p Creb1 , Addgene ID 154942, RRID:Addgene_154942) constructed after Creb1 cDNA introduction into a pCMVβ vector (Clontech, CA, #631719).

    Plasmid Preparation:

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. Vector construction and cell transfection The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
    Article Snippet: .. OHT treated EW7 melanocytes were co-transfected with eGFP-C1 (empty vector) and pcDNA3.1 (control), or eGFP-C1 and pcDNA3.1-α4 integrin wt (human, Addgene plasmid # 80016, gift from Chinten James Lim). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-x S/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis ( ) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production.
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-xS/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis (29) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. cell culture BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Control:

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. Vector construction and cell transfection The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: Immune-evasion of KRAS -mutant lung adenocarcinoma mediated by cAMP response element-binding protein
    Article Snippet: B16F10 p Kras G12C , PANO2 p Kras G12C and HEK293T p Kras G12C cells were generated after stable transfection of B16F10, PANO2 and HEK293T cells with plasmids eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372), eGFP.KRASG12C-2A.retro.puro (Addgene ID64373, RRID:Addgene_64373) and eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372) respectively as described elsewhere ( ). .. B16F10 p C and PANO2 p C were stably transfected with control (empty) overexpression vector eGFP.retro.puro (Addgene ID 64336, RRID:Addgene_64336) ( ). .. HEK293T p Kras G12C were stably transfected with p LUC (Cag.Luc.puro, Addgene ID 74409, RRID:Addgene_74409) or with plasmid enconding Creb1 (p Creb1 , Addgene ID 154942, RRID:Addgene_154942) constructed after Creb1 cDNA introduction into a pCMVβ vector (Clontech, CA, #631719).

    Article Title: Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin
    Article Snippet: .. OHT treated EW7 melanocytes were co-transfected with eGFP-C1 (empty vector) and pcDNA3.1 (control), or eGFP-C1 and pcDNA3.1-α4 integrin wt (human, Addgene plasmid # 80016, gift from Chinten James Lim). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-x S/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis ( ) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production.
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-xS/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis (29) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. cell culture BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Generated:

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. Vector construction and cell transfection The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-x S/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis ( ) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Article Title: FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes
    Article Snippet: To apply the adenovirus in animal experiments, the virus was purified by CsCl (Fisher Scientific, cat#BP1595-1) gradient centrifugation and dialysis. .. The Fam210a-flag overexpressed and empty vector control HEK293 cells were generated using pLJM-EGFP (Addgene #19319). ..

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production.
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-xS/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis (29) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. cell culture BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Incubation:

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Stable Transfection:

    Article Title: Immune-evasion of KRAS -mutant lung adenocarcinoma mediated by cAMP response element-binding protein
    Article Snippet: B16F10 p Kras G12C , PANO2 p Kras G12C and HEK293T p Kras G12C cells were generated after stable transfection of B16F10, PANO2 and HEK293T cells with plasmids eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372), eGFP.KRASG12C-2A.retro.puro (Addgene ID64373, RRID:Addgene_64373) and eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372) respectively as described elsewhere ( ). .. B16F10 p C and PANO2 p C were stably transfected with control (empty) overexpression vector eGFP.retro.puro (Addgene ID 64336, RRID:Addgene_64336) ( ). .. HEK293T p Kras G12C were stably transfected with p LUC (Cag.Luc.puro, Addgene ID 74409, RRID:Addgene_74409) or with plasmid enconding Creb1 (p Creb1 , Addgene ID 154942, RRID:Addgene_154942) constructed after Creb1 cDNA introduction into a pCMVβ vector (Clontech, CA, #631719).

    Over Expression:

    Article Title: Immune-evasion of KRAS -mutant lung adenocarcinoma mediated by cAMP response element-binding protein
    Article Snippet: B16F10 p Kras G12C , PANO2 p Kras G12C and HEK293T p Kras G12C cells were generated after stable transfection of B16F10, PANO2 and HEK293T cells with plasmids eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372), eGFP.KRASG12C-2A.retro.puro (Addgene ID64373, RRID:Addgene_64373) and eGFP.KRASG12C-2B.retro.puro (Addgene ID 64372, RRID:Addgene_64372) respectively as described elsewhere ( ). .. B16F10 p C and PANO2 p C were stably transfected with control (empty) overexpression vector eGFP.retro.puro (Addgene ID 64336, RRID:Addgene_64336) ( ). .. HEK293T p Kras G12C were stably transfected with p LUC (Cag.Luc.puro, Addgene ID 74409, RRID:Addgene_74409) or with plasmid enconding Creb1 (p Creb1 , Addgene ID 154942, RRID:Addgene_154942) constructed after Creb1 cDNA introduction into a pCMVβ vector (Clontech, CA, #631719).

    Retroviral:

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-x S/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis ( ) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production.
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-xS/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis (29) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. cell culture BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Expressing:

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-x S/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis ( ) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    Article Title: SYK Inhibition Induces Apoptosis in Germinal Center-Like B Cells by Modulating the Antiapoptotic Protein Myeloid Cell Leukemia-1, Affecting B-Cell Activation and Antibody Production.
    Article Snippet: Anti-Mcl-1 (S-19; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-2 (C-2; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-Bcl-xS/L (S-18; Santa Cruz Biotechnology Cat# sc-819 RRID:AB_2144105), anti-GAPDH (Sigma-Aldrich Cat# G9545 RRID:AB_796208), and anti-PARP (Cell Signaling Technology Cat# 9542 also 9542S, 9542L, 9542P RRID:AB_2160739) antibodies were used for immunoblotting and anti-hCD19 (HIB19; BD Biosciences Cat# 555409 RRID:AB_395809), anti-hMcl-1 (Sigma-Aldrich Cat# HPA008455 RRID:AB_1079334), and anti-STAT3 (K-15; Santa Cruz Biotechnology Cat# sc-483 RRID:AB_632441) Abs were used for immunohistology. .. Retroviral particles were generated with the following plasmids: the expression plasmid pBabe-Flag-hMcl-1, a gift from Roger Davis (29) (Addgene plasmid # 25371), the empty vector control plasmid pBabe-puro-IRES-EGFP, a gift from L. Miguel Martins (Addgene plasmid # 14430), the envelope-expressing plasmid pCMV-VSV-G, a gift from Bob Weinberg (Addgene plasmid # 8454), and the packaging plasmid pCL-Eco (Novus Biologicals). .. cell culture BL41, RAMOS, and BL2 Burkitt’s lymphoma cells were cultured in complete RPMI medium: RPMI-1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dominique Dutscher), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma).

    other:

    Article Title: CD24 suppresses malignant phenotype by downregulation of SHH transcription through STAT1 inhibition in breast cancer cells.
    Article Snippet: Empty vector control (eGFP-Control) and STAT1 plasmids (eGFPSTAT1) were purchased from Addgene (Cambridge, MA, USA).



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    TaKaRa egfp empty vector control
    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test